PERFF-seq

Transcript-specific enrichment enables profiling of rare cell states via scRNA-seq

Critical reagents

• Molecular Instruments HCR-FlowFISH
• 10x Genomics Flex scRNA-seq
• Thermo Fisher Scientific dsDNase

Key links

• Find the full work available on our bioRxiv preprint
• Read the step-by-step protocols.io workflow for PERFF-seq and cell/nuclei processing
• Reproduce all custom code and analyses from our Github Repository
• Access all raw and processed data available on GEO
• Find an easy-to-read digest of our results here
• Easily prepare buffers and reagents with the PERFF-seq benchside notes

Live sketch summary of Caleb’s talk at scgd24 by Alex Cagan

Contact the authors